Composite

Part:BBa_K4729704

Designed by: Yasoo Morimoto   Group: iGEM23_Marburg   (2023-10-10)


Ptac + VirG TiBo542 N80D + BBR1

a

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1741
    Illegal BamHI site found at 262
    Illegal BamHI site found at 1917
    Illegal BamHI site found at 2209
    Illegal XhoI site found at 227
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 111
    Illegal NgoMIV site found at 2054
  • 1000
    COMPATIBLE WITH RFC[1000]

References

Khan, S. R., Gaines, J., Roop, R. M., II, & Farrand, S. K. (2008). Broad-Host-Range Expression Vectors with Tightly Regulated Promoters and Their Use To Examine the Influence of TraR and TraM Expression on Ti Plasmid Quorum Sensing. In Applied and Environmental Microbiology (Vol. 74, Issue 16, pp. 5053–5062). American Society for Microbiology. https://doi.org/10.1128/aem.01098-08

Seiler, V. N. (2023). Improved inducible switches for the implementation of genetic tools in Sinorhizobium meliloti and Ensifer adhaerens (Master's thesis, Philipps-University Marburg)


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